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19-Mar-2025
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Arch Hellen Med, 42(3), May-June 2025, 330-340 REVIEW Application of CRISPR/Cas9 technology on research in diabetes mellitus and obesity I.A. Anastasiou, E. Rebelos, A. Tentolouri, E. Apostolopoulou, N. Tentolouris |
The clustered regularly interspaced short palindromic repeats (CRISPR) associated proteins (CRISPR/Cas9) genetic intervention method was one of the greatest scientific discoveries of the last decade. The highly efficient and precise ability to edit the genome with CRISPR/Cas9 technology has great therapeutic value and benefits, providing basic science with an additional research tool. CRISPR technology has been used mainly in cancer biology, virology, and basic cell biology; however, in diabetes research there has been limited work. One possible reason for this is that diabetes and obesity research is more complex because it involves many organs and cell types. However, many unanswered questions can still be answered using the CRISPR/Cas9 genetic intervention method. In this review, we summarize the current research data and results available using CRISPR/Cas9 and we try to provide an insight on how it can be used in diabetes and obesity research in the future.
Key words: Beta cells, Cas9, CRISPR/Cas, Diabetes mellitus, Obesity.
